sampling | Deep Sea News https://deepseanews.com All the news on the Earth's largest environment. Thu, 20 Nov 2014 12:00:03 +0000 en-US hourly 1 https://csrtech.com Putting snails in the microwave…for science! https://deepseanews.com/2014/11/putting-snails-in-the-microwave-for-science/ https://deepseanews.com/2014/11/putting-snails-in-the-microwave-for-science/#comments Thu, 20 Nov 2014 12:00:03 +0000 https://www.deepseanews.com/?p=53843 Yahoo answers tells me I shouldn’t put snails in the microwave, but this paper tells me otherwise: Galindo LA, Puillandre N, Strong EE, Bouchet P…

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Yahoo answers tells me I shouldn’t put snails in the microwave, but this paper tells me otherwise:

Galindo LA, Puillandre N, Strong EE, Bouchet P (2014) Using microwaves to prepare gastropods for DNA barcoding. Molecular Ecology Resources, 14(4): 700-705.

This paper is so simple, yet so epic in so many ways:

We have experimented with a method traditionally used to clean shells that involves placing the living gastropods in a microwave (MW) oven; the electromagnetic radiation very quickly heats both the animal and the water trapped inside the shell, resulting in separation of the muscles that anchor the animal to the shell. Done properly, the body can be removed intact from the shell and the shell voucher is preserved undamaged.

To reiterate: these researchers put snails in the microwave and got a paper out of it. Now of course, this is actually a brilliant method – the scientists stumbled across this quick fix because they need to preserve BOTH the shell and DNA from their specimens. With such thick shells, preservatives can’t get into the tissue very easily, and other methods (boiling the snails alive! or using chemical relaxants to pull out the muscle) are time consuming, sloooowwwwww, and downright dangerous:

To some extent, [these methods] can also represent a hazard (electrical drill and boiling water) on an unstable research vessel at sea.

Microwaves can zap lots of animals quickly and keep all their DNA intact!

(CC-licensed image from Flickr)
(CC-licensed image from Flickr)

This paper also wins for the most unnecessary use of acronyms, shortening the terms for microwaves (MW) and microwave ovens (MWO). So in everyday life I guess we can now further reduce MWs to a hand signal, and just say that we’re going to heat up our coffee in the “Muah”.

Don't try this with chickens (CC-licensed images from Flickr)
Don’t try this with chickens (CC-licensed images from Flickr)

 

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Gulf sampling, part deux https://deepseanews.com/2011/03/gulf-sampling-part-deux/ https://deepseanews.com/2011/03/gulf-sampling-part-deux/#comments Tue, 29 Mar 2011 03:21:49 +0000 https://www.deepseanews.com/?p=13317 My sporadic posting lately has been due to my ridiculous travel schedule – in the past 2 weeks, I’ve been to New York, Maine, San…

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My sporadic posting lately has been due to my ridiculous travel schedule – in the past 2 weeks, I’ve been to New York, Maine, San Diego, and now the Gulf of Mexico.  I’m currently on another short sampling trip, and at the end of the week I’ll be lecturing on a ‘Bioinformatics of Biodiversity’ workshop that we’re running for undergrads at Auburn University.

Fort Morgan, Alabama

Right now I’m headed along the coast from Dauphin Island, Alabama to (hopefully) Tallahassee Florida.  I’ve got two main goals on this trip.  First goal: to re-sample the five sites we are investigating around Dauphin Island.  Our pre-spill samples were collected in May 2010 before the oil hit shore, and while I’m down here I figured it would be good to get some ‘post-spill’ samples that represent the roughly the same time of year.  (The first lot of post-spill samples were collected in September, so we want to be rule out potential seasonal differences in beach communities).  Second goal: to collect samples where the oil didn’t reach.  For this, I’ll have to drive 90 minutes east of Panama City, towards Apalachee Bay on the other side of the peninsula.   All this driving is another reminder of just how far the slick reached – last fall I drove 1700 miles and didn’t reach an impact-free beach.

The Rental Car Samplemobile = pure awesomeness
Taxonomic and Molecular samples, collected by yours truly

Spring is a time of renewal and rebirth.  Right now it certainly feels like the Gulf is coming back to life, out of the (literal) blackness that followed the BP spill.  Last Autumn this coast was a ghost town everywhere I went – no cars, no tourists, only locals shaking their heads and venting their frustrations.  Needless to say, I was happily surprised to see Dauphin Island so bustling today; there were lots of tourists, families on the beach and patrons at most restaurants I passed.  I’m not saying everything is fixed – far from it – but Americans seem to be feeling confident enough to start resuming visits to the Gulf.  I haven’t seen any evidence of BP cleaning crews on the beaches, but I haven’t really seen any oil either (although if you dug down on Dauphin Island, there were brownish streaks of what looked like oil).  Tomorrow will be the real test, as I’m headed out to all the Florida beaches.  I’ll be sure to post some more updates later in the week.

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