To kick off WOD celebrations, we were super fortunate at Georgia Aquarium to get a short-notice visit from James Cameron’s Deepsea Challenger submersible this week, on it’s way to its new home at Woods Hole Oceanographic Institution.  If you remember, all of us at DeepSN wrote about the amazing achievements of Cameron’s team back in 2012 when they safely returned to the bottom of the Marianas Trench south of Guam for the first time since 1960 and only the second time ever (Piccard and Walsh being the first, in bathyscaphe Trieste). Read those posts here and here and here and here and here and here.   Several of the DC team were present at the festivities, as were some folks from WHOI including Dr. David Gallo and long time DSV Alvin pilot Anthony Tarantino.  It was awesome to have the sub there in the flesh, so to speak, and there was much rejoicing.

The first thing you notice about the sub is that it’s not as big as you might expect.  It’s sleek, uncluttered, and very very GREEN.  They’ve got a great rig for transporting it around and showing it off, including the training sphere (the real sphere is inside the faring at the right hand end of the image above, in between the blue post and the tyres you can see that protect the base.

The sphere itself is MINISCULE.  It’s hard for me to relate just how tiny it is, but here’s me holding my 18 month old daughter in front of it.  Just think, James Cameron is over 6 feet tall and had to be locked into that thing for over 7 hours, with 7 miles of water over his head.  It was not only an amazing engineering endeavour, but also an incredible human endurance feat.  While in the sphere, the only view out was between his knees and through the tiny port you can see in the door (about the size of a baseball)

One of the more interesting aspects of the subs design are the battery arrays, both in their type and arrangement.  Most subs use lead acid batteries, but to get the energy density and compactness they needed, the DC team used Lithium ion.  They’re arrayed in cells on the outside of the middle pod, separated by syntactic foam firewalls and are plumbed together with lots of tubing.  Tarantino told me that they had to it that way in case any one cell should melt or catch fire.  When I look at the arrays all I can think of is “wow, there’s a lot of little bits there that could break under that sort of pressure”; but obviously they designed it properly because it worked.

It was a great thrill to be able to examine the sub closely and to talk to those who were there on the day that the dive was made (although Cameron himself couldn’t be present).  There was an excited atmosphere among the staff and the guests alike as people peppered the DC team with questions about deepsea exploration.  I had an absolute ball and consider myself pretty lucky, since it stopped only in Dallas, Atlanta and DC on its way to Woods Hole.  In it’s new home, WHOI engineers will work alongside the DC team on technology transfer and training and then ultimately the sub should become part of the WHOI operations fleet.  That shouldn’t be too hard because the sub deploys with a standard deck crane and some cargo straps (!), and does’nt need fancy A-frames like many submersibles – another benefit of its lightweight design. With any luck the US deep submergence fleet will then double from 1 (Alvin) to 2!

Read more about the Deepsea Challenger and it’s mission here:

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Word on the street is that there are ~200 deep-sea biologists attending, and the one and only James Cameron will be delivering a plenary talk about his badass Deepsea Challenge submersible on Tuesday morning. Unfortunately I couldn’t attend this year, so I’ll be following along online whilst drowning my sorrows in Grey Goose martinis (and maybe inventing a twitter-based drinking game?).

Day 1 has already seen some über cool stuff, such as the new iDeep app being released for deep-sea taxonomy. What other exciting news will unfold??

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Despite a faulty hydraulics hampering sample collections, the Deepsea Challenger managed to grab half a sediment core – a cupful of muddy, watery ooze from the deepest point in the ocean:

“Jim recovered about 50 millileters of muddy seawater that I gleefully processed for culturing and for genomic studies,” Doug Bartlett, chief scientist for the DEEPSEA CHALLENGE project, said in an email to National Geographic News.

“Can’t wait to see what new critters (Bacteria, Archaea, and fungi) that we discover,” said Bartlett, a marine biologist at the Scripps Institution of Oceanography in San Diego, California.

Some might lament Cameron’s technical difficulties and shake their heads at the lost sampling opportunity. But even half a sediment core will reveal precious information about one of the last frontiers on earth. We have plenty to work with.

In molecular terms, 50 milliliters is a LOT of sample. Normally my lab protocols call for 200 microliters of mud for a single extraction of environmental DNA. So with his one cup of mud, James Cameron can do 250 DNA extractions–and you only need one or two extractions (maybe a few more, which are then concentrated and pooled if there isn’t a lot of DNA because of few animals or small amounts of tissue) before you can move forward and produce gene sequences, using high-throughput platforms such as the Illumina Hi-Seq.

So even with a single drop of sample, you can obtain hundreds of millions of DNA sequences from species inhabiting the Challenger Deep. And there’s no restriction to any particular taxonomic group. The power of DNA means that we will be able to characterize deep sea life across all known domains–bacteria, archaea, eukaryotes, and even viruses.

One of the first things to sequence will be ribosomal RNA, a conserved gene that essentially serves as a molecular barcode (since every cell needs its ribosomes to survive!) and allows us to place species on branches within the Tree of Life. By comparing ribosomal genes from the Challenger Deep to those from species that have already been studied, we’ll be able to place this deep-sea community in an evolutionary context and investigate how life might have evolved in the ocean depths. What other environments contain closely related species? How divergent are the ribosomal genes in the Mariana Trench (and from this, we can start guessing how long these trench communities have been isolated–if at all–from other deep sea habitats)? Is the Challenger Deep harboring any novel, undiscovered branches on the Tree of Life?

We’ll also get an environmental metagenome from this sequencing effort — randomly sequenced pieces of DNA representing every species’ genome lurking in that muddy sample. This will give us an expanded view compared to ribosomal genes, and we can start inferring things about community function. What type of genes are prevalent in the deepest, darkest ocean trench? The types of genes we find can tell us a lot about how a community survives (does it rely on scarce food sinking from above, or have species adapted to use alternative metabolic pathways such as chemosynthesis), and how an assemblage of organisms might inherently depend on each other to survive in an extreme environment. If the community in the Challenger Deep is not too complex (a handful of species, or a good pool of abundant ones) and the scientists at Scripps decide to sequence a LOT of DNA from this precious mud (a couple runs on the Illumina Hi-Seq can get you close to a billion DNA sequences), then it is possible that we might be able to assemble whole genomes from this random sample of mud. So instead of a ribosomal gene we’ll potentially have an entire genome as a molecular barcode for some microbial species–and for inferring how evolution happened in the deep-sea, a genome will give you a lot more information than just a short ribosomal sequence.

In addition to extracting DNA we can also take out the RNA and look at patterns in molecules such as mRNA (expressed transcripts of genes, if you remember back to high school biology). So in addition to finding out who’s there and what their genomes say they can do, RNA can tell us what these species might actually be doing. Remember that there’s a lot of “junk” DNA sitting around in any given genome (and you’ll get a lot of this information from a random environmental metagenome sequencing), so its always good to have additional information about what type of genes are being expressed. Now the pressure and temperature changes will have sent most species’ cellular machinery into overdrive during Cameron’s ascent to the surface, and we may get more of a “help, help , I’m dying” reaction from the community. Its always tricky to interpret gene expression. But in many ways, any data is good data. Gene expression from the Challenger Deep may tell us some very exciting things.

Doug Bartlett from Scripps also indicated plans to try and culture some microorganisms from Cameron’s sample — while there’s no guarantee of success (the pressure change and inherent difficulty in culturing microbes both present significant hurdles), any cultured species would be closely scrutinized and provide mountains of data for years to come. Not only could we sequence genomes from cultured species, but we could organize sophisticated experiments to figure out exactly what nutrients they need, their metabolic pathways, and novel compounds produced that all contribute to adaptation in the deep sea. We may soon have alien life growing in a Southern California lab!

These approaches alone will give you an unprecedented view into life in the Mariana Trench. But we can still do more with that half core.

Cameron noted that the ocean floor he saw was lunar-like, smooth and featureless–but that doesn’t mean the environment is exclusively the realm of microbes. In fact, we know that bigger (albeit still microscopic) species like foraminifera do live in the Challenger Deep (Todo et al., Science, 2005), and Cameron saw amphipods swimming around before the sample had even been returned to the lab. Which means we can probably get some cool visuals if we take another drop of mud and peer at its contents under the microscope. If there are amphipods and forams, there will be nematodes in Cameron’s sample. If nematodes can live ~3km deep within the fracture water of South African mines, they can certainly put up with a little bit of pressure and scraps of food in the Hadal zone. So yeah, we should be able to get some pictures like this (for inspiration and general awesomeness):

I’m not done yet. We can still get more from that core, including:

• Characterizing the chemical makeup of the sediment. This can be done via methods such as stable isotope analysis, and we can ask questions such as: Can we pinpoint the original source of the organic matter in the Challenger Deep? What type of food is available for organisms down there?  What does that say about conditions and species’ habitats in the deepest ocean trenches?
• Sediment geochemistry. What type of sediment is down there? Where did it come from (what continent or ocean region) and what trajectory might it have taken as it slowly sunk to the deepest depths?

All in all, Cameron’s sample will fundamentally contribute to our knowledge about some big questions in biology, such as:

Biological adaptations to life in extreme environments

Tim Shank, a deep-sea biologist at the Woods Hole Oceanographic Institution in Massachusetts, says that the waters above Challenger Deep are extremely unproductive; there is little algal life at the surface, and, therefore, less food is cycled down to deeper waters. “If it had been a trench with a productive water column, like the Kermadec Trench near New Zealand, I think he would have seen much more biology,” says Shank. However, sediment samples are certain to contain billions of microbes.

Insight into what life might be like on other planets:

The mud could contain exotic species of microbial life that may not only advance our understanding of the deep ocean but also help in the search for extraterrestrial life. For instance, scientists think Jupiter’s moon Europa could harbor a global ocean beneath its thick shell of ice—an ocean that, like Challenger Deep, would be lightless, near freezing, and home to areas of intense pressure. (See “Could Jupiter Moon Harbor Fish-Size Life?”)

So for those of you that scoffed at the botched sampling, there’s some serious scientific amazingness that awaits us in that half core of mud.

The post Challenger Deep: What we can learn from a single, half core of mud first appeared on Deep Sea News.